Isolation and characterization of a new cellulosome-producing Clostridium thermocellum strain

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Tachaapaikoon C., Kosugi A., Pason P., Waeonukul R., Ratanakhanokchai K., Kyu K.L., Arai T., Murata Y., Mori Y.

ปีที่เผยแพร่ (ค.ศ.): 2012

Volume number: 23

Issue number: 1

หน้าแรก: 57

หน้าสุดท้าย: 68

จำนวนหน้า: 12

นอก: 0923-9820

eISSN: 0923-9820

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84856647975&doi=10.1007%2fs10532-011-9486-9&partnerID=40&md5=7e1608976b0ba11839cd6caa08ae6e2f

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

The anaerobic thermophilic bacterium, Clostridium thermocellum, is a potent cellulolytic microorganism that produces large extracellular multienzyme complexes called cellulosomes. To isolate C. thermocellum organisms that possess effective cellulose-degrading ability, new thermophilic cellulolytic strains were screened from more than 800 samples obtained mainly from agriculture residues in Thailand using microcrystalline cellulose as a carbon source. A new strain, C. thermocellum S14, having high cellulose-degrading ability was isolated from bagasse paper sludge. Cellulosomes prepared from S14 demonstrated faster degradation of microcrystalline cellulose, and 3.4- and 5.6-fold greater Avicelase activity than those from C. thermocellum ATCC27405 and JW20 (ATCC31449), respectively. Scanning electron microscopic analysis showed that S14 had unique cell surface features with few protuberances in contrast to the type strains. In addition, the cellulosome of S14 was resistant to inhibition by cellobiose that is a major end product of cellulose hydrolysis. Saccharification tests conducted using rice straw soaked with sodium hydroxide indicated the cellulosome of S14 released approximately 1.5-fold more total sugars compared to that of ATCC27405. This newly isolated S14 strain has the potential as an enzyme resource for effective lignocellulose degradation. ฉ 2011 Springer Science+Business Media B.V.

คำสำคัญ

Hyper cellulolytic activity