Production and characterization of chitosan obtained from Rhizopus oryzae grown on potato chip processing waste
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Publication Details
Author list: Kleekayai T., Suntornsuk W.
Publisher: Springer
Publication year: 2011
Journal: World Journal of Microbiology and Biotechnology (0959-3993)
Volume number: 27
Issue number: 5
Start page: 1145
End page: 1154
Number of pages: 10
ISSN: 0959-3993
eISSN: 1573-0972
Languages: English-Great Britain (EN-GB)
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Abstract
Potato chip processing waste of trimmed potato, potato peel and substandard (low-quality) potato chips, obtained from a potato chip processing plant, were used as substrates for chitosan production from Rhizopus oryzae. It was cultured on each waste product at 30 ฑ 2ฐC and 70% moisture content for 21 days. Fermented potato peel had the highest yield after 5 days of fermentation. The cultivation condition of chitosan obtained from R. oryzae was optimum for a peel size of less than 6 mesh, 70% moisture content and a pH of 5. Furthermore, the best extraction condition was using 46% sodium hydroxide at 46ฐC for 13 h followed by 2% acetic acid at 95ฐC for 8 h. The maximum chitosan yield obtained by these conditions was 10. 8 g/kg substrate. Fungal chitosan properties were found to be 86-90% degree of deacetylation, molecular weight of 80-128 kDa and viscosity of 3. 1-6. 1 mPa s. Therefore, potato peel could be applied as a low cost substrate for chitosan production from R. oryzae. ฉ 2010 Springer Science+Business Media B.V.
Keywords
Fungal chitosan, Potato chip processing wastes, Rhizopus oryzae
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