Electrochemical genosensor for specific detection of the food-borne pathogen, Vibrio cholerae
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Author list: Low K.-F., Chuenrangsikul K., Rijiravanich P., Surareungchai W., Chan Y.-Y.
Publisher: Springer
Publication year: 2012
Journal: World Journal of Microbiology and Biotechnology (0959-3993)
Volume number: 28
Issue number: 4
Start page: 1699
End page: 1706
Number of pages: 8
ISSN: 0959-3993
eISSN: 1573-0972
Languages: English-Great Britain (EN-GB)
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Abstract
A disposable horseradish peroxidase (HRP)-based electrochemical genosensor was developed for chronoamperometric detection of single-stranded asymmetric lolB gene PCR amplicon (118 bp in length) of the food-borne pathogen, Vibrio cholerae. A two-step sandwich-type hybridization strategy using two specific probes was employed for specific detection of the target single-stranded DNA (ssDNA). The analytical performances of the detection platform have been evaluated using a synthetic ssDNA (ST3) which was identical to the target single-stranded amplicon and a total of 19 bacterial strains. Under optimal condition, ST3 was calibrated with a dynamic range of 0. 4883-15.6250 nM. By coupling asymmetric PCR amplification, the probe-based electrochemical genosensor was highly specific to the target organism (100% specificity) and able to detect as little as 0. 85 ng/μl of V. cholerae genomic DNA. © 2011 Springer Science+Business Media B.V.
Keywords
Asymmetric PCR amplicon, Carbon screen-printed electrode, Chronoamperometry, Probe-based sandwich-type hybridization, Vibrio cholerae
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