Preparation of chitosan nanoparticles for encapsulation and release of protein
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Publication Details
Author list: Jarudilokkul S., Tongthammachat A., Boonamnuayvittaya V.
Publisher: Springer
Publication year: 2011
Journal: Korean Journal of Chemical Engineering (0256-1115)
Volume number: 28
Issue number: 5
Start page: 1247
End page: 1251
Number of pages: 5
ISSN: 0256-1115
eISSN: 1975-7220
Languages: English-Great Britain (EN-GB)
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Abstract
Chitosan nanoparticles were prepared by ionic cross-linking with tripolyphosphate (TPP). The major effect on encapsulation and release of protein in chitosan-TPP nanoparticles was investigated in order to control the loading and release efficiency. A set of the same molecular weight (MW) proteins with different pI and a set of the same pI proteins with different MW were studied. The influence of protein concentration, pH of solution, and the activity of released protein were examined. It was found that the encapsulation efficiency (EE) of a set of the different MW protein decreased with increasing of MW of protein and protein concentration. The protein with having pI higher than pH of solution was attracted to the positively charged chitosan, resulting in increasing of EE. The release of protein from the nanoparticles showed that the protein release decreased with increasing of chitosan concentration, high MW protein, low pH, and less swelling of the particle. The released protein in chitosan-TPP matrix was still active in the buffer solution. ฉ 2011 Korean Institute of Chemical Engineers, Seoul, Korea.
Keywords
Controlled Release, Tripolyphosphate
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