Paenibacillus xylaniclasticus sp. nov., a xylanolytic-cellulolytic bacterium isolated from sludge in an anaerobic digester

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

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รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Tachaapaikoon C., Tanasupawat S., Pason P., Sornyotha S., Waeonukul R., Kyu K.L., Ratanakhanokchai K.

ผู้เผยแพร่: Springer

ปีที่เผยแพร่ (ค.ศ.): 2012

วารสาร: Journal of Microbiology (1225-8873)

Volume number: 50

Issue number: 3

หน้าแรก: 394

หน้าสุดท้าย: 400

จำนวนหน้า: 7

นอก: 1225-8873

eISSN: 1976-3794

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84863326181&doi=10.1007%2fs12275-012-1480-3&partnerID=40&md5=778bb0a7049b4969818c4d77824e3d2f

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

A mesophilic, facultative, anaerobic, xylanolytic-cellulolytic bacterium, TW1T, was isolated from sludge in an anaerobic digester fed with pineapple waste. Cells stained Gram-positive, were spore-forming, and had the morphology of straight to slightly curved rods. Growth was observed in the temperature range of 30 to 50ฐC (optimum 37ฐC) and the pH range of 6. 0 to 7. 5 (optimum pH 7. 0) under aerobic and anaerobic conditions. The strain contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The predominant isoprenoid quinone was menaquinone with seven isoprene units (MK-7). Anteiso-C15:0, iso-C16:0, anteiso-C17:0, and C16:0 were the predominant cellular fatty acids. The G+C content of the DNA was 49. 5 mol%. A phylogenetic analysis based on 16S rRNA showed that strain TW1T belonged within the genus Paenibacillus and was closely related to Paenibacillus cellulosilyticus LMG 22232T, P. curdlanolyticus KCTC 3759T, and P. kobensis KCTC 3761T with 97. 7, 97. 5, and 97. 3% sequence similarity, respectively. The DNA-DNA hybridization values between the isolate and type strains of P. cellulosilyticus LMG 22232T, P. curdlanolyticus KCTC 3759T, and P. kobensis KCTC 3761T were found to be 18. 6, 18. 3, and 18. 0%, respectively. The protein and xylanase patterns of strain TW1T were quite different from those of the type strains of closely related Paenibacillus species. On the basis of DNA-DNA relatedness and phenotypic analyses, phylogenetic data and the enzymatic pattern presented in this study, strain TW1T should be classified as a novel species of the genus Paenibacillus, for which the name Paenibacillus xylaniclasticus sp. nov. is proposed. The type strain is TW1T (=NBRC 106381T =KCTC 13719T =TISTR 1914T). ฉ 2012 The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.

คำสำคัญ

anaerobic digester, Paenibacillus xylaniclasticus, xylanolytic-cellulolytic bacterium