Microbial community structure and performance of an anaerobic reactor digesting cassava pulp and pig manure

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Panichnumsin P., Ahring B., Nopharatana A., Chaiprasert P.

ผู้เผยแพร่: IWA Publishing

ปีที่เผยแพร่ (ค.ศ.): 2012

วารสาร: Water Science & Technology (0273-1223)

Volume number: 66

Issue number: 7

หน้าแรก: 1590

หน้าสุดท้าย: 1600

จำนวนหน้า: 11

นอก: 0273-1223

eISSN: 1996-9732

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867638150&doi=10.2166%2fwst.2012.358&partnerID=40&md5=b779999c3f820cb9e26f3199bb1c2c74

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

Microbial community dynamics in response to changes in substrate types (i.e. pig manure (PM), cassava pulp (CP) and mixtures of PM and CP) were investigated in an anaerobic continuously stirred tank reactor (CSTR). Molecular identification of bacterial and archaeal domains were performed, using a 16S rDNA clone library with polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) screening and phylogenetic analysis. Analysis of bacterial clone libraries revealed that the differences in the community structure corresponded to the substrate types. However, the Bacteroidetes were the most abundant group in all substrates, followed by the Clostridia. With pure PM, the dominant bacterial groups were Bacteroidales, Clostridia and Paludibacter. With a co-substrate, at CP to PM (CP:PM) ratio of 50:50, the sequences analysis revealed the greatest diversity of bacterial communities at class level, and the sequences affiliated with Cytophaga sp. became an exclusive predominant. With CP alone, Bacteroides sp. was the dominant species and this reactor had the lowest diversity of bacteria. Archaea observed in the CSTR fed with all substrate types were Methanosaeta sp., Methanosaeta concilii and Methanospirillum hungatei. Among the Archaea, Methanosaeta sp. was the exclusive predominant. The relative distribution of Archaea also changed regarding to the substrate types. ฉ IWA Publishing 2012.

คำสำคัญ

Anaerobic digestion, Cassava pulp (Manihot esculenta Crantz), Clone library, Microbial community structure, Pig manure