Microbial community structure and performance of an anaerobic reactor digesting cassava pulp and pig manure
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Author list: Panichnumsin P., Ahring B., Nopharatana A., Chaiprasert P.
Publisher: IWA Publishing
Publication year: 2012
Journal: Water Science & Technology (0273-1223)
Volume number: 66
Issue number: 7
Start page: 1590
End page: 1600
Number of pages: 11
ISSN: 0273-1223
eISSN: 1996-9732
Languages: English-Great Britain (EN-GB)
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Abstract
Microbial community dynamics in response to changes in substrate types (i.e. pig manure (PM), cassava pulp (CP) and mixtures of PM and CP) were investigated in an anaerobic continuously stirred tank reactor (CSTR). Molecular identification of bacterial and archaeal domains were performed, using a 16S rDNA clone library with polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) screening and phylogenetic analysis. Analysis of bacterial clone libraries revealed that the differences in the community structure corresponded to the substrate types. However, the Bacteroidetes were the most abundant group in all substrates, followed by the Clostridia. With pure PM, the dominant bacterial groups were Bacteroidales, Clostridia and Paludibacter. With a co-substrate, at CP to PM (CP:PM) ratio of 50:50, the sequences analysis revealed the greatest diversity of bacterial communities at class level, and the sequences affiliated with Cytophaga sp. became an exclusive predominant. With CP alone, Bacteroides sp. was the dominant species and this reactor had the lowest diversity of bacteria. Archaea observed in the CSTR fed with all substrate types were Methanosaeta sp., Methanosaeta concilii and Methanospirillum hungatei. Among the Archaea, Methanosaeta sp. was the exclusive predominant. The relative distribution of Archaea also changed regarding to the substrate types. ฉ IWA Publishing 2012.
Keywords
Anaerobic digestion, Cassava pulp (Manihot esculenta Crantz), Clone library, Microbial community structure, Pig manure
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