Elicitors (chitosan and hyaluronic acid) affect protocorm-like body formation in hybrid Cymbidium
Journal article
Authors/Editors
Strategic Research Themes
No matching items found.
Publication Details
Author list: Da Silva J.A.T., Uthairatanakij A., Obsuwan K., Shimasaki K., Tanaka M.
Publisher: Global Science Books
Publication year: 2013
Volume number: 7
Issue number: 1
Start page: 77
End page: 81
Number of pages: 5
ISSN: 1749-0294
eISSN: 1749-0294
Languages: English-Great Britain (EN-GB)
Abstract
Protocorm-like body (PLB) production from hybrid Cymbidium Twilight Moon 'Day Light' was examined on a new medium, Teixeira Cymbidium (TC) medium in response to chitosan and other elicitors. TCPLB, the control medium specific for induction of PLBs, contained a unique set of micro- and macro-nutrients, 0.1 mg/1 a-naphthaleneacetic acid and 0.1 mg/1 kinetin, 2 g/1 tryptone and 20 g/1 sucrose, and solidified with 8 g/1 Bacto agar. Crude deacetylated chitin (i.e., chitosan), chitosan and hyaluronic acid (HA) had a pronounced effect on PLB formation, depending on the concentration, when added to TC medium without plant growth regulators (PGRs). In all cases, the performance was never better than TC, although 0.1-1.0 mg/1 chitosan or HA produced more PLBs/PLB and a greater fresh weight than TC basal medium without PGRs. This indicates that both these elicitors could induce PLBs and thus acted as phytohormones. Peroxidase activity, a signal of stress induction, increased in PLB extracts of PLBs grown on medium containing chitosan and HA, peaking when these elicitors were supplied at 50 mg/1. ฉ 2012 Global Science Books.
Keywords
Basal medium, Hyaluronic acid or hyaluronan, PLB, Teixeira Cymbidium (TC) medium
Contact Information