Efficient saccharification for non-treated cassava pulp by supplementation of Clostridium thermocellum cellulosome and Thermoanaerobacter brockii β-glucosidase
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Author list: Vaithanomsat P., Kosugi A., Apiwatanapiwat W., Thanapase W., Waeonukul R., Tachaapaikoon C., Pason P., Mori Y.
Publisher: Elsevier
Publication year: 2013
Journal: Bioresource Technology (0960-8524)
Volume number: 132
Start page: 383
End page: 386
Number of pages: 4
ISSN: 0960-8524
Languages: English-Great Britain (EN-GB)
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Abstract
Cassava pulp containing 60% starch and 20% cellulose is a promising renewable source for bioethanol. The starch granule was observed to tightly bind cellulose fiber. To achieve an efficient degradation for cassava pulp, saccharification tests without pre-gelatinization treatment were carried out using combination of commercial α-amylase with cellulosome from Clostridium thermocellum S14 and β-glucosidase (rCglT) from Thermoanaerobacter brockii. The saccharification rate for cassava pulp was shown 59% of dry matter. To obtain maximum saccharification rate, glucoamylase (GA) from C. thermocellum S14 was supplemented to the combination. The result showed gradual increase in the saccharification rate to 74% (dry matter). Supplementation of GA to the combination of commercial α-amylase, cellulosome and rCglT is powerful method for efficient saccharification of cassava pulp without pretreatment. © 2012 Elsevier Ltd.
Keywords
saccharification
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