Optimized simultaneous saccharification and co-fermentation of rice straw for ethanol production by Saccharomyces cerevisiae and Scheffersomyces stipitis co-culture using design of experiments
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Author list: Suriyachai N., Weerasaia K., Laosiripojana N., Champreda V., Unrean P.
Publisher: Elsevier
Publication year: 2013
Journal: Bioresource Technology (0960-8524)
Volume number: 142
Start page: 171
End page: 178
Number of pages: 8
ISSN: 0960-8524
Languages: English-Great Britain (EN-GB)
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Abstract
Herein an ethanol production process from rice straw was optimized. Simultaneous saccharification and co-fermentation (SSCF) using Saccharomyces cerevisiae and Scheffersomyces stipitis co-culture was carried out to enhance ethanol production. The optimal saccharification solid loading was 5%. Key fermentation parameters for co-culture including cell ratio, agitation rate and temperature was rationally optimized using design of experiment (DoE). Optimized co-culture conditions for maximum ethanol production efficiency were at S. cerevisiae:. S. stipitis cell ratio of 0.31, agitation rate of 116. rpm and temperature of 33.1. ฐC. The optimized SSCF process reached ethanol titer of 15.2. g/L and ethanol yield of 99% of theoretical yield, consistent with the DoE model prediction. Moreover, SSCF process under high biomass concentration resulted in high ethanol concentration of 28.6. g/L. This work suggests the efficiency and scalability of the developed SSCF process which could provide an important basis for the economic feasibility of ethanol production from lignocelluloses. ฉ 2013 Elsevier Ltd.
Keywords
Co-culture, Design of Experiment, Simultaneous saccharification and co-fermentation (SSCF) optimization
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