Thermodynamically unfavorable DNA hybridizations can be made to occur by a water to ice phase change
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Publication Details
Author list: Krissanaprasit A., Guajardo C., Somasundrum M., Surareungchai W.
Publisher: Elsevier
Publication year: 2013
Journal: Cryobiology: International Journal of Low Temperature Biology and Medicine (0011-2240)
Volume number: 66
Issue number: 1
Start page: 81
End page: 84
Number of pages: 4
ISSN: 0011-2240
Languages: English-Great Britain (EN-GB)
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Abstract
In an apparent contradiction to Debye-Hückel theory, it was possible to hybridize DNA in solutions of Milli-Q water (resistivity>18MΩcm-1) containing no added ions. This was demonstrated by hybridizing four bi-complementary DNA sequences to form an 'X' shape, as indicated by acrylamide gel electrophoresis. The requirement for hybridization was that a water-to-ice phase change should occur. Comparative experiments, using freezing by liquid nitrogen and thawing at different temperatures, showed that hybridization could take place during either the freezing or thawing process provided either was slow enough. We speculate that the low solubility of DNA in ice creates liquid inclusions of extremely high DNA and counter-ion concentration prior to complete freezing, and that hence in these inclusions hybridization was actually in accordance with Debye-Hückel theory. © 2012 Elsevier Inc.
Keywords
Debye-H�ckel theory, Electrostatic repulsion, Gibbs free energy, Ice, Nucelic acid
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