Polysaccharide extraction from Spirulina sp. and its antioxidant capacity
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Author list: Chaiklahan R., Chirasuwan N., Triratana P., Loha V., Tia S., Bunnag B.
Publisher: Elsevier
Publication year: 2013
Journal: International Journal of Biological Macromolecules (0141-8130)
Volume number: 58
Start page: 73
End page: 78
Number of pages: 6
ISSN: 0141-8130
Languages: English-Great Britain (EN-GB)
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Abstract
To optimize polysaccharide extraction from Spirulina sp., the effect of solid-to-liquid ratio, extraction temperature and time were investigated using Box-Behnken experimental design and response surface methodology. The results showed that extraction temperature and solid-to-liquid ratio had a significant impact on the yield of polysaccharides. A polysaccharides yield of around 8.3% dry weight was obtained under the following optimized conditions: solid-to-liquid ratio of 1:45, temperature of 90. ฐC, and time of 120. min. The polysaccharide extracts contained rhamnose, which accounted for 53% of the total sugars, with a phenolic content of 45. mg GAE/g sample. ฉ 2013 Elsevier B.V.
Keywords
Antioxidant activity, Polysaccharide
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