Semiquantitative expression of anthocyanin biosynthetic genes in a vanda hybrid-pale mauve (V. teres ื V. hookeriana)
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Publication Details
Author list: Junka N., Kanlayanarat S., Buanong M., Wongchaochant S., Wongs-Aree C.
Publisher: International Society for Horticultural Science (ISHS)
Publication year: 2012
Volume number: 943
Start page: 131
End page: 136
Number of pages: 6
ISBN: 9789066050204
ISSN: 0567-7572
eISSN: 2406-6168
Languages: English-Great Britain (EN-GB)
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Abstract
The expression of four genes involved in the anthocyanin biosynthetic pathway, phenylalanine ammonia-lyase [PAL], chalcone synthase [CHS], flavanone 3-hydroxylase [F3H] and dihydroflavonol 4-reductase [DFR], was spatially investigated in root, stem, leaf, unopened-floret, half-full bloom floret and full bloom floret of a Vanda hybrid-pale mauve orchid (V. teres ื V. hookeriana) using reverse transcriptase polymerase chain reaction (RT-PCR). The competitive RT-PCR showed that PAL was highly expressed in all vegetative parts and all three developmental stages of the orchid florets. CHS was sharply expressed in the florets during development, compared to the vegetative organs. With similar to CHS but even more, the F3H expression was significant in floral parts including unopenedfloret, and floret at half-full bloom and full bloom stages. The expression of DFR fluctuated in the plant organs which was low in the leaves.
Keywords
Anthocyanin biosynthetic pathway, Reverse transcriptase polymerase chain reaction (RT-PCR)
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