Biocatalytic synthesis of starch esters by immobilized lipase on magnetic microparticles
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Publication Details
Author list: Prasertpornsakun N., Raita M., Laosiripojana N., Champreda V.
Publisher: Taylor & Francis: STM, Behavioural Science and Public Health Titles
Publication year: 2015
Journal: Bioscience, Biotechnology and Biochemistry (0916-8451)
Volume number: 79
Issue number: 11
Start page: 1750
End page: 1758
Number of pages: 9
ISSN: 0916-8451
Languages: English-Great Britain (EN-GB)
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Abstract
Enzymatic esterification is an efficient approach for modifying starch to functionalized biomaterials. In this study, conversion of cassava starch to fatty acid acyl esters using immobilized Thermomyces lanuginosus lipase on Fe3O4 microparticles modified with 3-aminopropyltriethoxysilane and covalently linked by 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide and N-hydroxysuccinimide (Fe3O4-AP-ED-lipase) in a solvent-free system was studied. An optimized reaction containing 5% w/v gelatinized starch, 1% v/v Triton X-100, and 1% w/v biocatalyst with 2.5% w/v of fatty acids (palmitic, oleic, or linoleic acid) resulted in esterified products with a degree of substitution (DS) of 0.12-0.14, while a slightly lower DS was observed using crude palm fatty acid distillate as the acyl donor with 42.9-59.6% recovery yield. Increasing DS led to lower glass transition temperature and higher viscosity of the esterified products. The enzyme showed high operational stability with 85% retaining in activity after recycling in three consecutive batches with simple separation by magnetization, leading to improved process economics. ฉ 2015 Japan Society for Bioscience, Biotechnology, and Agrochemistry.
Keywords
Fatty acid, Immobilized lipase, Magnetic microparticle, Starch ester
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