Development of ochratoxin A detection based on electrochemical sensor using Auball labels

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Pinijsuwan S., Rijiravanich P., Surareungchai W.

ผู้เผยแพร่: Universiti Sains Malaysia

ปีที่เผยแพร่ (ค.ศ.): 2016

Volume number: 12

Issue number: 5

หน้าแรก: 332

หน้าสุดท้าย: 338

จำนวนหน้า: 7

นอก: 2231-7538

eISSN: 2231-7538

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85014718492&partnerID=40&md5=0420c08bbd2c821c5e32dd1ba53be26a

ภาษา: English-Great Britain (EN-GB)

บทคัดย่อ

Aims: The present study was aimed to develop a highly sensitive and rapid method for ochratoxin A (OTA) detection. Methodology and results: In this study, an electrochemical peptidesensor for OTA detection was developed using numerous number of Au particles coated on the surface of silica particle (Au-ball). Moreover, this assay was performed in 384 well plate, so the multiple detections was done. The synthesized silica particle was spherical in shape and size was 275ฑ17 nm. After coated with Au layer, size of Au-ball was 280ฑ14 nm. Au particles loaded can be taken up resulting in approx. 1ื107 Au3+ molecules per silica particle. Moreover, the optimization conditions were studied. The limit of detection of this assay showed as low as 2 ppb. Conclusion, significance and impact study: This platform showed rapid, sensitive and specific to ochratoxin A detection. In spite of these advantages, this Au-ball based peptidesensors is suitable to use in the detection of ochratoxin A contaminated in coffee or seed industry.

คำสำคัญ

Au-ball labels, Coffee, Ochratoxin A, Peptide