Development of ochratoxin A detection based on electrochemical sensor using Auball labels
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Publication Details
Author list: Pinijsuwan S., Rijiravanich P., Surareungchai W.
Publisher: Universiti Sains Malaysia
Publication year: 2016
Volume number: 12
Issue number: 5
Start page: 332
End page: 338
Number of pages: 7
ISSN: 2231-7538
eISSN: 2231-7538
Languages: English-Great Britain (EN-GB)
Abstract
Aims: The present study was aimed to develop a highly sensitive and rapid method for ochratoxin A (OTA) detection. Methodology and results: In this study, an electrochemical peptidesensor for OTA detection was developed using numerous number of Au particles coated on the surface of silica particle (Au-ball). Moreover, this assay was performed in 384 well plate, so the multiple detections was done. The synthesized silica particle was spherical in shape and size was 275ฑ17 nm. After coated with Au layer, size of Au-ball was 280ฑ14 nm. Au particles loaded can be taken up resulting in approx. 1ื107 Au3+ molecules per silica particle. Moreover, the optimization conditions were studied. The limit of detection of this assay showed as low as 2 ppb. Conclusion, significance and impact study: This platform showed rapid, sensitive and specific to ochratoxin A detection. In spite of these advantages, this Au-ball based peptidesensors is suitable to use in the detection of ochratoxin A contaminated in coffee or seed industry.
Keywords
Au-ball labels, Coffee, Ochratoxin A, Peptide
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