The effect of light-emitting diode irradiation at different wavelengths on calcification of osteoblast-like cells in 3D culture
Conference proceedings article
ผู้เขียน/บรรณาธิการ
กลุ่มสาขาการวิจัยเชิงกลยุทธ์
ไม่พบข้อมูลที่เกี่ยวข้อง
รายละเอียดสำหรับงานพิมพ์
รายชื่อผู้แต่ง: Chintavalakorn R., Tanglitanont T., Khantachawana A., Viravaidya-Pasuwat K., Santiwong P.
ปีที่เผยแพร่ (ค.ศ.): 2015
Volume number: 2015-November
หน้าแรก: 3513
หน้าสุดท้าย: 3516
จำนวนหน้า: 4
ISBN: 9781424492718
นอก: 1557-170X
ภาษา: English-Great Britain (EN-GB)
บทคัดย่อ
This study aimed to investigate the effect of four different light-emitting diode (LED) wavelengths on calcification and proliferation of osteoblast-like cells in vitro. MC3T3-E1 cells were seeded within three-dimensional collagen scaffolds and irradiated daily by LED light with peak emission wavelengths of 630-, 680-, 760- and 830-nm at constant fluency of 3.1 J/cm2 (irradiance intensity 2 mW/cm2). Cultures were measured for calcium content at day 0, 7, 14, 21, 28, 35 and 42. The significant enhancement in calcium content was observed at the early stage of culture (days 7 and 14) (p<0.05). After that, the calcium content of irradiated groups was similar to that of the controls group. This suggests the transient effect of light irradiation on osteoblastic cell calcification. Only 680-nm irradiated samples revealed a significant enhancement of calcium content until the late stages of culture (from days 21 to 42) (p<0.001). The cyclin D mRNA expression that was investigated 3 hours after stimulation at day3 also show that the 680-nm LED irradiation can enhance cyclin D expression more than others. For enhancing bone mineralization, LED irradiation at the 680-nm is more effective than those at 630-, 760- and 830-nm. Further studies should be investigated in order to obtain the most effective parameters of LLLI on bone regeneration in clinical setting. ฉ 2015 IEEE.
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