Novel trifunctional xylanolytic enzyme Axy43A from Paenibacillus curdlanolyticus strain B-6 exhibiting endo-xylanase, β-D-xylosidase, and arabinoxylan arabinofuranohydrolase activities

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Author list: Teeravivattanakit T., Baramee S., Phitsuwan P., Waeonukul R., Pason P., Tachaapaikoon C., Sakka K., Ratanakhanokchai K.

Publisher: American Society for Microbiology

Publication year: 2016

Journal: Applied and Environmental Microbiology (0099-2240)

Volume number: 82

Issue number: 23

Start page: 6942

End page: 6951

Number of pages: 10

ISSN: 0099-2240

eISSN: 1098-5336

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84996619315&doi=10.1128%2fAEM.02256-16&partnerID=40&md5=b9a29bd872ebaeac24a97a07ccf725e2

Languages: English-Great Britain (EN-GB)

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Abstract

The axy43A gene encoding the intracellular trifunctional xylanolytic enzyme from Paenibacillus curdlanolyticus B-6 was cloned and expressed in Escherichia coli. Recombinant PcAxy43A consisting of a glycoside hydrolase family 43 and a family 6 carbohydrate- binding module exhibited endo-xylanase, β-xylosidase, and arabinoxylan arabinofuranohydrolase activities. PcAxy43A hydrolyzed xylohexaose and birch wood xylan to release a series of xylooligosaccharides, indicating that PcAxy43A contained endo-xylanase activity. PcAxy43A exhibited β-xylosidase activity toward a chromogenic substrate, p-nitrophenyl-β-D-xylopyranoside, and xylobiose, while it preferred to hydrolyze long-chain xylooligosaccharides rather than xylobiose. In addition, surprisingly, PcAxy43A showed arabinoxylan arabinofuranohydrolase activity; that is, it released arabinose from both singly and doubly arabinosylated xylose, α-L-Araf-(1→2)-D-Xylp or α-L-Araf-(1→3)-D-Xylp and α-L-Araf-(1→2)-[α-L-Araf-(1→3)]-β-DXylp. Moreover, the combination of PcAxy43A and P. curdlanolyticus B-6 endo-xylanase Xyn10C greatly improved the efficiency of xylose and arabinose production from the highly substituted rye arabinoxylan, suggesting that these two enzymes function synergistically to depolymerize arabinoxylan. Therefore, PcAxy43A has the potential for the saccharification of arabinoxylan into simple sugars for many applications. © 2016, American Society for Microbiology. All Rights Reserved.

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