Characterization and high-quality draft genome sequence of Herbivorax saccincola A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Aikawa S., Baramee S., Sermsathanaswadi J., Thianheng P., Tachaapaikoon C., Shikata A., Waeonukul R., Pason P., Ratanakhanokchai K., Kosugi A.

ผู้เผยแพร่: Elsevier GmbH

ปีที่เผยแพร่ (ค.ศ.): 2018

Volume number: 41

Issue number: 4

หน้าแรก: 261

หน้าสุดท้าย: 269

จำนวนหน้า: 9

นอก: 0723-2020

eISSN: 0723-2020

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042360404&doi=10.1016%2fj.syapm.2018.01.010&partnerID=40&md5=2e043356f2d211c7efb4beb4030174ff

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

An anaerobic, cellulolytic-xylanolytic bacterium, designated strain A7, was isolated from a cellulose-degrading bacterial community inhabiting bovine manure compost on Ishigaki Island, Japan, by enrichment culture using unpretreated corn stover as the sole carbon source. The strain was Gram-positive, non-endospore forming, non-motile, and formed orange colonies on solid medium. Strain A7 was identified as Herbivorax saccincola by DNA-DNA hybridization, and phylogenetic analysis based on 16S rRNA gene sequences showed that it was closely related to H. saccincola GGR1 (= DSM 101079T). H. saccincola A7 (= JCM 31827 = DSM 104321) had quite similar phenotypic characteristics to those of strain GGR1. However, the optimum growth of A7 was at alkaline pH (9.0) and 55 ฐC, compared to pH 7.0 at 60 ฐC for GGR1, and the fatty acid profile of A7 contained 1.7-times more C17:0 iso than GGR1. The draft genome sequence revealed that H. saccincola A7 possessed a cellulosome-like extracellular macromolecular complex, which has also been found for Clostridium thermocellum and C. clariflavum. H. saccincola A7 contained more glycoside hydrolases (GHs) belonging to GH families-11 and -2, and more diversity of xylanolytic enzymes, than C. thermocellum and C. clariflavum. H. saccincola A7 could grow on xylan because it encoded essential genes for xylose metabolism, such as a xylose transporter, xylose isomerase, xylulokinase, and ribulose-phosphate 3-epimerase, which are absent from C. thermocellum. These results indicated that H. saccincola A7 has great potential as a microorganism that can effectively degrade lignocellulosic biomass. ฉ 2018 Elsevier GmbH

คำสำคัญ

Alkaliphile, Cellulolytic bacterium, Herbivorax saccincola