Performance and microbial consortium structure in simultaneous removal of sulfur and nitrogen compounds under micro-oxygenated condition

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Charoensuk P., Thongnueakhaeng W., Chaiprasert P.

ผู้เผยแพร่: Center for Environmental and Energy Research and Studies

ปีที่เผยแพร่ (ค.ศ.): 2019

Volume number: 16

Issue number: 10

หน้าแรก: 5767

หน้าสุดท้าย: 5782

จำนวนหน้า: 16

นอก: 1735-1472

eISSN: 1735-1472

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057332822&doi=10.1007%2fs13762-018-2132-x&partnerID=40&md5=085af490b33d411849f77025fc585d48

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

Effects of COD/SO4 2− and SO4 2−/NH4 + ratios on simultaneous removal of sulfate- and ammonium-rich synthetic wastewaters were investigated under micro-oxygenated condition (dissolved oxygen concentration at 0.10–0.15 mg/L). Lactate was served as carbon source to generate COD/SO4 2− ratios of 2.0, 2.5, 3.0 3.5 and 4.0. The batch experimental results indicated that the highest sulfate (72.1%) and ammonium (62.8%) removal efficiencies could be reached at COD/SO4 2− ratio of 4.0. The main metabolic products were elemental sulfur (0.63 g S0/g SO4 2−–Sadded) and nitrogen gas (0.57 g N2/g NH4 +–Nadded). Subsequently, various SO4 2−/NH4 + ratios (0.5, 1.0, 1.5, 2.0 and 2.5) were performed at controlled COD/SO4 2− ratio of 4.0. The highest SO4 2−/NH4 + ratio of 2.5 provided 76.6 and 72.8% sulfate and ammonium removal efficiencies, respectively, and also reached the highest yield of elemental sulfur and nitrogen gas of 0.68 g S0/g SO4 2−–Sadded and 0.66 g N2/g NH4 +–Nadded, respectively. Microbial consortium structure providing the highest removal efficiencies was consequently analyzed using Illumina sequencing and polymerase chain reaction-denaturing gradient gel electrophoresis approaches. Taxonomic assignments demonstrated that Proteobacteria (46%), Firmicutes (15%), and Bacteroidetes (14%) were the most abundant phyla. Almost core genera analysis with two distinguished approaches demonstrated similar results. Aside from microbial community analysis, quantitative real-time polymerase chain reaction was used to validate existing abundance of aforementioned seven dominant microorganisms. The nitrous oxide reductase gene was shown the most abundance (~ 108 copies/µL) which plays a crucial role for simultaneous removal of sulfur and nitrogen compounds. © 2018, Islamic Azad University (IAU).

คำสำคัญ

Illumina sequencing, Micro-oxygenation, Polymerase chain reaction-denaturing gradient gel electrophoresis, Quantitative real-time polymerase chain reaction, Simultaneous biological removal, Sulfate and ammonium