Performance and microbial consortium structure in simultaneous removal of sulfur and nitrogen compounds under micro-oxygenated condition
Journal article
Authors/Editors
Strategic Research Themes
No matching items found.
Publication Details
Author list: Charoensuk P., Thongnueakhaeng W., Chaiprasert P.
Publisher: Center for Environmental and Energy Research and Studies
Publication year: 2019
Volume number: 16
Issue number: 10
Start page: 5767
End page: 5782
Number of pages: 16
ISSN: 1735-1472
eISSN: 1735-1472
Languages: English-Great Britain (EN-GB)
View in Web of Science | View on publisher site | View citing articles in Web of Science
Abstract
Effects of COD/SO4 2− and SO4 2−/NH4 + ratios on simultaneous removal of sulfate- and ammonium-rich synthetic wastewaters were investigated under micro-oxygenated condition (dissolved oxygen concentration at 0.10–0.15 mg/L). Lactate was served as carbon source to generate COD/SO4 2− ratios of 2.0, 2.5, 3.0 3.5 and 4.0. The batch experimental results indicated that the highest sulfate (72.1%) and ammonium (62.8%) removal efficiencies could be reached at COD/SO4 2− ratio of 4.0. The main metabolic products were elemental sulfur (0.63 g S0/g SO4 2−–Sadded) and nitrogen gas (0.57 g N2/g NH4 +–Nadded). Subsequently, various SO4 2−/NH4 + ratios (0.5, 1.0, 1.5, 2.0 and 2.5) were performed at controlled COD/SO4 2− ratio of 4.0. The highest SO4 2−/NH4 + ratio of 2.5 provided 76.6 and 72.8% sulfate and ammonium removal efficiencies, respectively, and also reached the highest yield of elemental sulfur and nitrogen gas of 0.68 g S0/g SO4 2−–Sadded and 0.66 g N2/g NH4 +–Nadded, respectively. Microbial consortium structure providing the highest removal efficiencies was consequently analyzed using Illumina sequencing and polymerase chain reaction-denaturing gradient gel electrophoresis approaches. Taxonomic assignments demonstrated that Proteobacteria (46%), Firmicutes (15%), and Bacteroidetes (14%) were the most abundant phyla. Almost core genera analysis with two distinguished approaches demonstrated similar results. Aside from microbial community analysis, quantitative real-time polymerase chain reaction was used to validate existing abundance of aforementioned seven dominant microorganisms. The nitrous oxide reductase gene was shown the most abundance (~ 108 copies/µL) which plays a crucial role for simultaneous removal of sulfur and nitrogen compounds. © 2018, Islamic Azad University (IAU).
Keywords
Illumina sequencing, Micro-oxygenation, Polymerase chain reaction-denaturing gradient gel electrophoresis, Quantitative real-time polymerase chain reaction, Simultaneous biological removal, Sulfate and ammonium
Contact Information