Efficient biological pretreatment and bioconversion of corn cob by the sequential application of a Bacillus firmus K-1 cellulase-free xylanolytic enzyme and commercial cellulases

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Fatmawati N.V., Ketbot P., Phitsuwan P., Waeonukul R., Tachaapaikoon C., Kosugi A., Ratanakhanokchai K., Pason P.

ผู้เผยแพร่: Springer

ปีที่เผยแพร่ (ค.ศ.): 2021

วารสาร: Applied Microbiology and Biotechnology (0175-7598)

Volume number: 105

Issue number: 11

หน้าแรก: 4589

หน้าสุดท้าย: 4598

จำนวนหน้า: 10

นอก: 0175-7598

eISSN: 1432-0614

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85106410273&doi=10.1007%2fs00253-021-11308-9&partnerID=40&md5=4ec8b4a7f1933da710c212049cdc135e

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

Abstract: We used agricultural residue, corn cob, with biorefinery and bioeconomy concepts. At short-time cultivation in corn cob (12 h), Bacillus firmus K-1 produced cellulase-free xylanolytic enzyme, with xylooligosaccharides (XOSs), X5 and X6, as the main products, which can be used in a variety of applications. The xylanolytic enzyme produced from B. firmus K-1 effectively degraded xylan in corn cob, which was examined by chemical composition, scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FTIR). After cultivation, the xylan contained in the corn cob residue was decreased (as biological pretreatment), causing morphological and structural changes, including creating porosity and increasing the surface area and the exposure of cellulose of pretreated corn cob. These results lead to an improvement of cellulose access by cellulases. Commercially available cellulases, Accellerase® 1500 and Cellic® CTec2, yielded significantly higher glucose concentrations from pretreated corn cob compared to untreated corn cob. After saccharification, the lignin-rich corn cob residue can be used as a raw material for other purposes. Moreover, the B. firmus cells, with a low risk to human health, can be used in some applications. This study presents an efficient method for producing high-value-added products from agricultural residue (corn cob) through biological processes which are environmentally friendly and economically viable. Key points: • High-value-added products were efficiently produced from corn cob by B. firmus K-1. • After biological pretreatment by B. firmus K-1, cellulase can better reach cellulose. • XOSs and cellulose-derived glucose were the main products from corn cob. © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

คำสำคัญ

Cellulase-free xylanolytic enzyme, Xylooligosaccharide