Comparative genomics of Edwardsiella ictaluri revealed four distinct host-specific genotypes and thirteen potential vaccine candidates
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Author list: Machimbirike V.I., Uthaipaisanwong P., Khunrae P., Dong H.T., Senapin S., Rattanarojpong T., Sutheeworapong S.
Publisher: Elsevier
Publication year: 2021
Journal acronym: Genomics
Volume number: 113
Issue number: 4
Start page: 1976
End page: 1987
Number of pages: 12
ISSN: 0888-7543
Languages: English-Great Britain (EN-GB)
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Abstract
Edwardsiella ictaluri has been considered an important threat for catfish aquaculture industry for more than 4 decades and an emerging pathogen of farmed tilapia but only 9 sequenced genomes were publicly available. We hereby report two new complete genomes of E. ictaluri originated from diseased hybrid red tilapia (Oreochromis sp.) and striped catfish (Pangasianodon hypophthalmus) in Southeast Asia. E. ictaluri species has an open pan-genome consisting of 2615 core genes and 5592 pan genes. Phylogenetic analysis using core genome MLST (cgMLST) and ANI values consistently placed E. ictaluri isolates into 4 host-specific genotypes. Presence of unique genes and absence of certain genes from each genotype provided potential biomarkers for further development of genotyping scheme. Vaccine candidates with high antigenic, solubility and secretion probabilities were identified in silico from the core genes. Microevolution within the species is brought about by bacteriophages and insertion elements and possibly drive host adaptation. © 2021 Elsevier Inc.
Keywords
Biomarkers, Complete genome, Host-specific genotypes, Pangenome analysis, Vaccine candidates
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