Applicability of infectivity assay for the quantification of infectious human adenovirus genotype 5 in UV-irradiated wastewater

บทความในวารสาร


ผู้เขียน/บรรณาธิการ


กลุ่มสาขาการวิจัยเชิงกลยุทธ์


รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่งRattanakul S., Oguma K.

ผู้เผยแพร่IWA Publishing

ปีที่เผยแพร่ (ค.ศ.)2021

Volume number19

Issue number1

หน้าแรก67

หน้าสุดท้าย78

จำนวนหน้า12

นอก1477-8920

eISSN1996-7829

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85102000036&doi=10.2166%2fWH.2020.224&partnerID=40&md5=38d459b254b7435908a2033c2064acd0

ภาษาEnglish-Great Britain (EN-GB)


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บทคัดย่อ

The use of infectivity assays in domestic wastewater samples is limited because of the concerns around cytotoxicity to host cells, thus, the UV inactivation efficiency of human adenoviruses (HAdVs) in wastewater remains unclear. In this study, a human adenovirus genotype five (HAdV-5) host cell line (A549 cells) was incubated with wastewater at varied dilutions from 1:1.5 to 1:4 (the ratio of wastewater to a mixture of wastewater and the cell culture medium) and the cytotoxicity was assessed by the cell morphology and viability. No change was observed in either cell viability or morphology in comparison with control samples, even at lowest dilution of 1:1.5, indicating the dilution allowed infectivity assays. The minimal degree of dilution to avoid cytotoxicity may differ with different water matrix. Consequently, the technique was applied to quantify spiked HAdV-5 after the UV disinfection of wastewater. A significant increase in UV disinfection efficiency was noted in wastewater and hydroxyl radicals (OH•) produced by the photosensitization of dissolved organic matter were suggested to be responsible for the enhancement. This study indicated that dilution can be a simple solution to avoid cytotoxicity, and UV inactivation may be enhanced in wastewater due to OH• radicals produced by UV radiation. © 2021 The Authors.


คำสำคัญ

adenovirusInfectivity assayUV disinfection


อัพเดทล่าสุด 2023-17-10 ถึง 07:36