Genomic and transcriptomic analysis identified novel putative cassava lncRNAs involved in cold and drought stress

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Suksamran, Rungaroon; Saithong, Treenut; Thammarongtham, Chinae; Kalapanulak, Saowalak;

ผู้เผยแพร่: MDPI

ปีที่เผยแพร่ (ค.ศ.): 2020

Volume number: 11

Issue number: 4

นอก: 20734425

eISSN: 2073-4425

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85082791997&doi=10.3390%2fgenes11040366&partnerID=40&md5=0690be1671e557cbdd6fa93ffe89dae2

ภาษา: English-Great Britain (EN-GB)

ดูในเว็บของวิทยาศาสตร์ | ดูบนเว็บไซต์ของสำนักพิมพ์ | บทความในเว็บของวิทยาศาสตร์

บทคัดย่อ

Long non-coding RNAs (lncRNAs) play important roles in the regulation of complex cellular processes, including transcriptional and post-transcriptional regulation of gene expression relevant for development and stress response, among others. Compared to other important crops, there is limited knowledge of cassava lncRNAs and their roles in abiotic stress adaptation. In this study, we performed a genome-wide study of ncRNAs in cassava, integrating genomics-and transcriptomics-based approaches. In total, 56,840 putative ncRNAs were identified, and approximately half the number were verified using expression data or previously known ncRNAs. Among these were 2229 potential novel lncRNA transcripts with unmatched sequences, 250 of which were differentially expressed in cold or drought conditions, relative to controls. We showed that lncRNAs might be involved in post-transcriptional regulation of stress-induced transcription factors (TFs) such as zinc-finger, WRKY, and nuclear factor Y gene families. These findings deepened our knowledge of cassava lncRNAs and shed light on their stress-responsive roles. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

คำสำคัญ

Cold and drought stress, Comparative approach, LncRNA