Genomic and transcriptomic analysis identified novel putative cassava lncRNAs involved in cold and drought stress
Journal article
Authors/Editors
Strategic Research Themes
Publication Details
Author list: Suksamran, Rungaroon; Saithong, Treenut; Thammarongtham, Chinae; Kalapanulak, Saowalak;
Publisher: MDPI
Publication year: 2020
Volume number: 11
Issue number: 4
ISSN: 20734425
eISSN: 2073-4425
Languages: English-Great Britain (EN-GB)
View in Web of Science | View on publisher site | View citing articles in Web of Science
Abstract
Long non-coding RNAs (lncRNAs) play important roles in the regulation of complex cellular processes, including transcriptional and post-transcriptional regulation of gene expression relevant for development and stress response, among others. Compared to other important crops, there is limited knowledge of cassava lncRNAs and their roles in abiotic stress adaptation. In this study, we performed a genome-wide study of ncRNAs in cassava, integrating genomics-and transcriptomics-based approaches. In total, 56,840 putative ncRNAs were identified, and approximately half the number were verified using expression data or previously known ncRNAs. Among these were 2229 potential novel lncRNA transcripts with unmatched sequences, 250 of which were differentially expressed in cold or drought conditions, relative to controls. We showed that lncRNAs might be involved in post-transcriptional regulation of stress-induced transcription factors (TFs) such as zinc-finger, WRKY, and nuclear factor Y gene families. These findings deepened our knowledge of cassava lncRNAs and shed light on their stress-responsive roles. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords
Cold and drought stress, Comparative approach, LncRNA
ติดต่อสอบถามเพิ่มเติมได้ที่