Characterization of a thermophilic facultatively anaerobic bacterium Paenibacillus sp. strain DA-C8 that exhibits xylan degradation under anaerobic conditions

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Chhe C., Uke A., Baramee S., Tachaapaikoon C., Pason P., Waeonukul R., Ratanakhanokchai K., Kosugi A.

ผู้เผยแพร่: Elsevier

ปีที่เผยแพร่ (ค.ศ.): 2021

วารสาร: Journal of Biotechnology (0168-1656)

Volume number: 342

หน้าแรก: 64

หน้าสุดท้าย: 71

จำนวนหน้า: 8

นอก: 0168-1656

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118348676&doi=10.1016%2fj.jbiotec.2021.10.008&partnerID=40&md5=d992274d3064ab8ee6dbc0be738446d8

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

The screening, identification, and study of the functional properties of cellulolytic xylanolytic bacteria are crucial for the construction of applicable bioprocesses. The thermophilic facultatively anaerobic, xylanolytic bacterial strain DA-C8 (=JCM34211=DSM111723) exhibiting efficient xylan degradation was newly isolated from compost. Strain DA-C8 completely degraded 1% beechwood xylan within 4 days under anaerobic conditions. By 16S rRNA gene sequence homology and phylogenetic tree analysis, strain DA-C8 was closely related to Paenibacillus cisolokensis and Xylanibacillus composti; however, the average nucleotide identity and digital DNA-DNA hybridization values based on genome information and the carbon source utilization properties indicated that strain DA-C8 belongs to Paenibacillus rather than Xylanibacillus. The gene numbers of xylanase and endoglucanase of strain DA-C8 and X. composti were not different; however, strain DA-C8 had higher abundance of α-L-arabinofuranosidase, β-xylosidase, and β-glucosidase than X. composti. Strain DA-C8 showed decreased xylan and corn hull degradation abilities and growth on xylan medium under aerobic conditions. Quantitative PCR showed high expression of xylan and cellulose degradation genes under anaerobic conditions, but the genes were repressed under aerobic conditions, indicating that strain DA-C8 controls polysaccharide degradation depending on the aeration conditions. Strain DA-C8 is a new species of Paenibacillus with a unique polysaccharide degradation system. © 2021 Elsevier B.V.

คำสำคัญ

Anaerobic growth condition, Facultative anaerobic bacteria, Paenibacillus