Antioxidant and enzyme-inhibitory activities of bromelain-derived rice bran protein hydrolysates and ultrafiltered peptide fractions

Journal article

Authors/Editors

Strategic Research Themes

Sustainable Bioeconomy (Strategic Research Themes)

Publication Details

Author list: Khothuta, P.; Selamassakul, O.; Kaisangsri, N.; Kaprasob, R.; Uthairatanakij, A.; Laohakunjit, N.

Publisher: Taylor and Francis Group

Publication year: 2025

Journal: International Journal of Food Properties (1094-2912)

Volume number: 28

Issue number: 1

Start page: 2571166

ISSN: 1094-2912

eISSN: 1532-2386

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-105018668972&doi=10.1080%2F10942912.2025.2571166&partnerID=40&md5=618b089c22bb24eef7968ece4d2c81bd

Languages: English-Great Britain (EN-GB)

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Abstract

Bioactive peptides derived from proteins are widely used as functional foods and dietary supplements. This study focused on producing bioactive peptides from rice bran (Khao Dawk Mali 105). The enzymatic bromelain rice bran protein hydrolyzate (eb-RBPH) was separated into five fractions by ultrafiltration: < 1 kDa, 1–3 kDa, 3–5 kDa, 5–10 kDa, and > 10 kDa. The 1–3 kDa (F4) fraction showed the greatest DPPH and ABTS radical-scavenging activities (IC50 values of 696.02 and 444.13 µg protein/mL, respectively), elastase inhibition (IC50 = 51.90 µg protein/mL), collagenase inhibition (IC50 = 17.84 µg protein/mL), and gelatinase inhibition (IC50 = 27.72 µg protein/mL), followed by the < 1 kDa (F5) fraction and the unfractionated segment (eb-RBPH). Moreover, the 1–3 kDa fraction showed no cytotoxic effects toward African green monkey kidney fibroblasts, human immortal keratinocyte cells, or mouse subcutaneous connective tissue, suggesting its suitability for use as a functional ingredient. Chemometric analysis confirmed that the antioxidant and anti-aging properties of the eb-RBPH and its fractions were associated with bioactive peptides. © 2025, Published with license by Taylor & Francis Group, LLC. © 2025 Phathamon Khothuta, Orrapun Selamassakul, Nattapon Kaisangsri, Ratchadaporn Kaprasob, Apiradee Uthairatanakij and Natta Laohakunjit.

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