Physicochemical characteristics and textural parameters of restructured pork steaks hydrolysed with bromelain
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Publication Details
Author list: Saengsuk N., Laohakunjit N., Sanporkha P., Kaisangsri N., Selamassakul O., Ratanakhanokchai K., Uthairatanakij A.
Publisher: Elsevier
Publication year: 2021
Journal: Food Chemistry (0308-8146)
Volume number: 361
ISSN: 0308-8146
Languages: English-Great Britain (EN-GB)
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Abstract
Enzymatic tenderisation including bromelain enhances underused cuts of meat in emerged restructuring technology. Physicochemical and textural characteristics of restructured pork steak hydrolysed with bromelain for masticatory dysfunction people were evaluated. Restructured pork steak treated with bromelain at 0.05 and 0.1% (w/w) was hydrolysed at 50 °C for 0, 3, 6, 9 and 12 min. The cooking losses of 0.05% (w/w) bromelain for 0, 3 and 6 min were lower than 0.1% (w/w) bromelain samples. The ΔE increased after increasing the enzyme concentration and hydrolysis time. Bromelain-treated samples at higher concentrations showed lower WBSF, KSF and TPA parameters, but cohesiveness of 0.05% (w/w) had higher than 0.1% (w/w) bromelain samples. Total protein, sarcoplasmic protein solubility, TCA-soluble peptide, total collagen and soluble collagen contents were the highest in 0.1% (w/w) bromelain-treated samples for 12 min (P < 0.05). According to SDS-PAGE and SEM, various proteins in the enzyme-treated samples were degraded. © 2021 Elsevier Ltd
Keywords
Protein solubility, Restructured pork steak, Soluble collagen, Tenderness
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