Impact of electron beam irradiation on the chlorophyll degradation and antioxidant capacity of mango fruit

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Publication Details

Author list: Nguyen T.T., Uthairatanakij A., Srilaong V., Laohakunjit N., Kato M., Jitareerat P.

Publisher: Elsevier

Publication year: 2021

Journal: Postharvest Biology and Technology (0925-5214)

Volume number: 64

Issue number: 1

ISSN: 0925-5214

eISSN: 1873-2356

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85100426547&doi=10.1186%2fs13765-021-00592-8&partnerID=40&md5=a10c6450cb2f62f8fc598c0f61de6c2e

Languages: English-Great Britain (EN-GB)

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Abstract

At the present, the mechanism of chlorophyll degradation in response to ionizing irradiation in harvested fruits have not been examined. To understand the effect of electron beam (E-beam) irradiation on the chlorophyll degrading pathway in relation to chlorophyll degrading enzymes activity, reactive oxygen species (ROS) and antioxidant capacities of harvested mangoes stored at 13 °C for 16 days were studied. E-beam-treated fruit significantly suppressed the activities of chlorophyll degrading enzymes especially pheophytinase (PPH) and chlorophyll degrading peroxidase (Chl-POX) in the late stage of storage. This resulted in the chlorophyll content being maintained. However, E-beam irradiation did not affect the activities of chlorophyllase (Chlase) and magnesium de-chelatase (MD). The respiration rate, ethylene production, ROS accumulation (hydrogen peroxide [H2O2] and superoxide radical [O−.2]) immediately increased after E-beam treatment, following which they significantly decreased in comparison to the control. E-beam treatment enhanced the fruit’s antioxidant capacity by activating the activities of catalase (CAT) and ascorbate peroxidase (APX) and glutathione (GSH) content, and inactivated the activity of superoxide dismutase (SOD). Further, it did not affect the activity of glutathione reductase (GR) and glutathione disulfide (GSSG), vitamin C content, or total phenolic content. These results imply that E-beam treatment has the potential to delay chlorophyll degradation by suppressing the Chl-POX and PPH activities as well as reduce ROS production via CAT, APX, and SOD activities and GSH content. © 2021, The Author(s).

Keywords

Antioxidant capacity, Chlorophyll degradation, Ionizing irradiation, Reactive oxygen species